Information on the diversity and transmission of those viruses are limited in ethiopia. Maize chlorotic mottle virus how is maize chlorotic mottle. Concentration of maize chlorotic mottle virus increased in mixed infections with maize dwarf mosaic virus, strain b. Althoughthe genetic basis of resistance to potyviruses is well understood in maize, little was known about resistance to mcmv. Transmission of maize chlorotic mottle virus by chrysomelid beetles. Maize chlorotic mottle virus mcmv infection causes growth abnormalities in maize. Pdf first report of maize chlorotic mottle virus and maize lethal. Complete nucleotide sequence of a maize chlorotic mottle. Maize chlorotic mottle virus mcmv, the only member of the genus machlomovirus in the family t ombusviridae, can infect various crops and lead to typical mild mosaic symptoms 28. Get a printable copy pdf file of the complete article 1. For this purpose, visual detection of maize chlorotic mottle virus was. Maize lethal necrosis mln is a disease of maize caused by coinfection of maize with maize chlorotic mottle virus mcmv and one of several viruses from the potyviridae, such as sugarcane mosaic.
Item 33 indian peanut clump virus item 34 maize chlorotic mottle virus item 35 pea earlybrowning virus item 36 tomato brown rugose fruit virus item 37 tomato leaf curl new delhi virus item 38 zucchini green mottle mosaic virus 2 regioncountries of distribution of the following seven quarantine pests will be updated. Maize chlorotic mottle virus mcmv combines with a potyvirus in. The complete nucleotide sequence of the maize chlorotic mottle virus mcmv genome has been determined to be 4437 nucleotides. In december 20, unusually severe chlorotic mottle symptoms and pale green streaks were observed in local varieties mudishi 1 and 2, bambou, kasayi, h614, h6, and mugamba and exotic varieties h520, h624, h403, hdk8031, and zm607 in beni, lubero, and. Our catalog 2020 can now be downloaded as pdf file. Seed maize produced in commercial nurseries in hawaii was harvested from symptomatic plants naturally infected by mcmv. Maize lethal necrosis agricultural research council. Realtime taqman rtpcr for detection of maize chlorotic. The disease is caused by a combination of two viruses, maize chlorotic mottle virus mcmv and sugarcane mosaic virus scmv, a pathogen prevalent in many parts of kenya affecting cereal crops. Maize chlorotic mottle virus causes corn lethal necrosis disease, and can be transmitted via infected maize seeds.
Maize chlorotic mottle virus, antiserum, corn, leaves, necrosis, nucleotide sequences, sequence analysis, virion, china abstract. Maize chlorotic mottle virus mcmv is a small, plussense single stranded rna virus of the genus machlomo virus, family tombusviridae. Gene functions of strains of maize chlorotic mottle virus, which comprises the monotypic genus machlomovirus, have not been previously identified. Most of these images are 1200 x 784 pixels and saved qith quality 12 maximum from photoshop. How the pathogen survives and the primary source of inoculum is unknown. The disease caused severe economic losses in the midwest and southern united states during the 1960s to 1970s.
The positive samples were further identified by rtpcr method and the 711 bp size target bands were amplified specifically. Mln is caused by coinfection of maize with the emergent maize chlorotic mottle virus mcmv and any of several maize infecting potyviruses endemic in east africa and worldwide. Monoclonal antibodybased serological methods for maize. Detection and characterization of maize chlorotic mottle. Categorization of maize chlorotic mottle virus mcmv00 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience.
Pdf characterization of maize mirnas in response to. Mcmv does not infect dicots but has a wide monocot host range with at least 19 grass species including cultivated ones, such as barley. On the basis of significant amino acid sequence homology in the viral polymerase, the machlomovirus genus has been placed within the family tombusviridae. Continuing to use this website means you agree to our use of cookies. Seed transmission was found by elisa and confirmed by symptomatology and mechanical transmission in 17 of 42 000 plants from 25 seed lots submitted by.
Notes on taxonomy and nomenclature top of page maize chlorotic mottle virus mcmv is the type and only species of the machlomovirus genus lommel, 1995. Jiang x q, meinke l j, wright r j, wilkinson d r, campbell j e, 1992. Apr 20, 2017 maize lethal necrosis disease mlnd develops from synergistic coinfection by sugarcane mosaic virus and maize chlorotic mottle virus. Maize chlorotic mottle virus is the third plant virus responsible for initial spread of the in kansas since known to be vectored by 0. Transmission of maize chlorotic mottle virus by chrysomelid. In the natural host, viral particles can be produced at 12 mg per gram of. Maize chlorotic mottle virus mcmv, an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. The primers and probe sequences in this kit have 100% homology with over 95% of reference. The concentration of maize chlorotic mottle virus mcmv was up to 5. The rna of maize chlorotic mottle virus the essential virus in. Recombinase polymerase amplification assay for rapid.
It has been reported in 37 states located in most regions of the country, including hawaii. Mcmv was first described in maize from peru in 1974 castillo and hebert, 1974 and thereafter was reported on maize plants in the united states and. The complete nucleotide sequence 4436 nt of a yunnan. We report that, like other tombusvirids, mcmv rna contains a capindependent translation element cite in its 3. First report of maize chlorotic mottle virus infecting. Maize lethal necrosis mln is emergent in east africa, first reported in 2011 in kenya, and is devastating to maize production in the region. The complete nucleotide sequence of pepper mottle virus genomic rna. We do not use these to store personal information about you. It suggests integrated practices for successful management of maize cholorotic mottle and identifies areas of future research needed to gain a fuller understanding of maize chlorotic mottle. Maize dwarf mosaic virus mdmv, or sugarcane mosaic virus scmv. The symptoms of md m and scm occur first in the younge st leaves as an irregular, lig ht or darkgreen. Maize chlorotic mottle virus mcmv was first reported in maize in china in 2009. Transcriptome sequencing was conducted to compare the global gene expression of mcmvinoculated plants with that of mockinoculated plants. Maize chlorotic mottle virus mcmv is transmitted by after acquisition.
The interactions of maize chlorotic mottle virus with potyviridae viruses causes an aggressive synergistic viral condition maize lethal necrosis. Maize chlorotic mottle virus mcmv is one of the co. Maize chlorotic mottle virus descriptions of plant viruses. Based on previous editions produced by cimmyt maize. Maize lethal necrosis was first identified in the usa in 1976 niblett and caflin, 1978. Maize chlorotic mottle virus exhibits low divergence between. Machlomovirus is a plant virus with icosahedral virions of 30 nm in diameter containing a singlestranded positivesense rna of. The viral genome has four long open reading frames orfs which could encode polypeptides of 31. First report of maize chlorotic mottle virus and maize lethal necrosis on maize in ethiopia. The maize lethal necrosis disease mlnd is a result of a combination of two viruses, the maize chlorotic mottle virus mcmov and any of the cereal viruses in the potyviridae group, like the sugarcane mosaic virus scmv, wheat streak mosaic virus wsmv or maize dwarf mosaic virus mdmv.
Viruses free fulltext nitric oxide as a downstream. Detection and identification of maize chlorotic mottle. Gbt 318102015 detection and identification of maize. We propose that mcmv, and some other positive strand rna viruses. General information about maize mottle chlorotic stunt virus mmcsv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Cowpea chlorotic mottle virus, known by the abbreviation ccmv, is a virus that specifically infects the cowpea plant, or blackeyed pea. Maize chlorotic mottle virus exhibits low divergence. In this study mutagenesis of the seven genes encoded in maize chlorotic mottle virus mcmv showed that the genes with positional and sequence similarity to their homologs in viruses of related tombusvirid genera had similar functions. Studies were conducted on kauai, hawaii to identify potential aboveground arthropod vectors of maize chlorotic mottle virus mcmv, to survey for plants serving as reservoirs for mcmv and also to survey for associated viruses in maize zea mays that might cause corn lethal necrosis disease. Symptoms of maize chlorotic dwarf on a young corn leaf r. Host range of mcdv was studied by exposing gramineae test spp.
If maize plants exhibit a rapid onset of necrosis followed by rapid plant death, it is likely that they are infected with both mcmv and a maize infecting potyvirus. Top of page maize chlorotic mottle virus mcmv is the type and only species of the. The complete nucleotide sequence of the maize chlorotic mottle virus genome. Negative control for maize chlorotic mottle virus mcmv. Exogenous 2,4epibrassinolide bl or brassinazole brz applications. The images also appear when selecting from individual virus names. However, the frequent association of maize chlorotic dwarf virus with such symptoms suggests that, in most cases, this disease is maize chlorotic dwarf and not corn stunt. If maize plants exhibit a rapid onset of necrosis followed by rapid plant death, it is likely that they are infected with both mcmv and a maize.
Maize dwarf mosaic of maize in the united states is caused by either of two major viruses. Item 33 indian peanut clump virus item 34 maize chlorotic mottle virus item 35 pea earlybrowning virus item 36 tomato brown rugose fruit virus item 37 tomato leaf curl new delhi virus item 38 zucchini green mottle mosaic virus 2 regioncountries of distribution. Further characterization of maize chlorotic mottle virus. The primerdesign genesig kit for maize chlorotic mottle virus mcmv genomes is designed for the in vitro quantification of mcmv genomes. Further characterization of maize chlorotic mottle virus and its. Leaves of corn plants inoculated early three to seven leaf stage with both maize chlorotic mottle virus and maize dwarf mosaic virus turn chlorotic then necrotic. This publication describe maize chlorotic mottle, the pathogen, vectors and virus transmission, and the disease epidemiology and symptoms. Maize chlorotic mottle virus in hawaiiangrown maize. Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed. Wft to transmit mcmv, imported maize seeds from thailand were germinated in an insect. Crop science abstract maize responses to a severe isolate. Partial and complete genome sequence data for maize viruses were obtained, including african isolates of maize chlorotic mottle virus and sugarcane mosaic virus and ohio isolates of the major u.
Diverse chromosomal locations of quantitative trait loci. In order to determine the ability of western flower thrips frankliniella occidentalis. The rna of maize chlorotic mottle virus the essential. This is one of the most widely distributed and important corn virus diseases in the u. Studies on maize streak virus and maize mottlechlorotic.
Transmission of maize chlorotic mottle virus by chrysomelid beetles article pdf available in phytopathology 687 january 1978 with 152 reads how we measure reads. Gb318102015detection and identification of maize chlorotic mottle virus. This negative control is intended for use in elisa test formats. The double infection of the two viruses gives rise to. For this purpose, a realtime taqman rtpcr procedure for efficient detection of mcmv was developed. The disease causes symptoms ranging from leaf tissue mottling and malformed ears to premature plant death.
Formation of organized structures from protein subunits in vitro bancroft, j. Maize chlorotic mottle virus mcmv is a positivesense singlestranded rna virus, and the sole member of the machlomovirus genus in the tombusviridae family. In nebraska greenhouse studies, the seeds were planted and 14 dayold seedlings were assayed for mcmv. Maize mottle chlorotic stunt virus mmcsv was prevalent in 99. It remains a challenge to detect this virus to prevent its introduction, infection and its transmission throughout fields. The complete nucleotide sequence of the maize chlorotic mottle.
Isometric virus particles 30 nm in diameter were found in. Pdf first report of maize chlorotic mottle virus and. This species also adults of both species overwinter and appear in the field transmits cocksfoot mottle virus cmv 17 and phleum early in the corngrowing season. Mcmv transmission studies used six arthropod species found in mcmvinfected maize fields peregrinus. The results of incidence ratings to assess the host response of maize zea mays l. In this study we further analyzed the epidemiology of mcmv and corn lethal necrosis disease clnd in china. A maize lethal necrosis disease was observed in yunnan province of china. The complete sequence of soybean chlorotic mottle virus dna and the identification of a novel promoter. Coinfection, maize chlorotic mottle virus mcmv, sugarcane mosaic virus scmv.
Data analyses showed that brassinosteroid brassociated genes were upregulated after mcmv infection. Pdf first report of maize chlorotic mottle virus on sweet corn in. If you desire to recieve a personal copy with price information. Maize chlorotic mottle virus mcmv is the only identified member of the genus machlomovirus in the family tombusviridae king et al. As a mixed infection with wheat streak mosaic virus wsmv, sugarcane mosaic virus, or maize dwarf mosaic virus, it induces a synergism referred to as corn lethal necrosis. The viri ons of this singlestranded rna virus are isometric, and the singlecomponent particles have a smooth spherical or hexagonal shape scheets 2010. Jan 09, 2020 maize chlorotic mottle virus mcmv combines with a potyvirus in maize lethal necrosis disease mlnd, an emerging disease worldwide that often causes catastrophic yield loss. No attempts were made to characterize and identify scmv and mcmv. Coat protein gene maize chlorotic mottle virus techne qpcr test 150 tests for general laboratory and research use only quantification of maize chlorotic mottle. Maize chlorotic mottle virus induces changes in host plant. Maize chlorotic mottle virus mcmv combines with a potyvirus in maize lethal necrosis disease mlnd, an emerging disease worldwide that often causes catastrophic yield loss.
Pathogen maize chlorotic mottle virus is the only species in the genus machlomovirus family tombusviridae. Pdf in february 2014, a severe disease on maize zea mays l. To determine the distribution of viruses in maize in china, we collected 171 maize leaf samples with viruslike symptoms between 2009 and 20. Isometric virus particles 30 nm in diameter were found in infected maize leaf tissues. Maize chlorotic mottle virus resembles a number of other viruses that have host ranges confined to the gramineae, high infectivity titres, high thermal inactivation points and isometric particles of c.
Machlomovirus is a genus of plant viruses, in the family tombusviridae. Pdf transcripts of a maize chlorotic mottle virus cdna. Maize lethal necrosis disease mlnd develops from synergistic coinfection by sugarcane mosaic virus and maize chlorotic mottle virus. The leaves of infected plants develop yellow spots, hence the name chlorotic. The virus complex causes a severe systemic necrosis which culminates in death of the plant niblett and claflin, 1978.
The synergistic infection of maize chlorotic mottle virus mcmv and sugarcane mosaic virus scmv causes maize lethal necrosis, with considerable losses to global maize production. Maize chlorotic dwarf of maize is caused by maize chlorotic dwarf virus mcdv. A study of the selfassembly process in a small spherical virus. Maize chlorotic mottle virus mcmv causes corn lethal necrosis disease, and can be transmitted through infected maize seeds. Ijms free fulltext characterization of maize mirnas in.
Maize lethal necrosis viruses and other maize viruses in. Maize chlorotic mottle scholarspace at university of hawaii. Elisa, diseased maize plant samples reacted positively with the antiserum of maize chlorotic mottle virus mcmv. The virus particles, about 750 nm in length, are shadowed with palladium. Pdf recombinase polymerase amplification assay for rapid. Characterization of maize mirnas in response to synergistic. The virus occurs in south central nebraska, north central kansas, and south america. Infectious clones development and transmission biology of maize chlorotic mottle virus. The double infection of the two viruses gives rise to what. It remains a challenge to detect this virus in the seeds to prevent its introduction and infection. Maize lethal necrosis is one of the most devastating diseases of maize causing yield losses reaching up to 90% in subsaharan africa. Detection and identification of maize chlorotic mottle virus. The elisa results showed that the two seedlings reacted positively with antibody against maize chlorotic mottle virus mcmv.
Maize chlorotic mottle virus mcmv is the type species of the genus machlomovirus. To inform resistance strategies, we characterized the translation initiation mechanism of mcmv. Analysis of gene functions in maize chlorotic mottle virus. The disease is caused by a combination of maize chlorotic mottle virus mcmv and any one of cereal viruses in the potyviridae group such as sugarcane mosaic virus. Maize chlorotic mottle virus mcmv is the only member of the machlomovirus genus in the family tombusviridae. Although major yield losses in field dent corn due to mcdv have not been.
Pdf maize lethal necrosis mln is a disease of maize caused by the combination of maize chlorotic mottle virus mcmv and any of the viruses. Pdf transmission of maize chlorotic mottle virus by. Exogenous 2,4epibrassinolide bl or brassinazole brz. Maize chlorotic mottle virus is a plant pathogenic virus. The kit is designed to have the broadest detection profile possible whilst remaining specific to the mcmv genome. The complete nucleotide sequence of the maize chlorotic. Similar to its brother virus, cowpea mosaic virus cpmv, ccmv is produced in high yield in plants. Mcmv has been reported to be transmitted mainly by maize thrips frankliniella williamsi and. Visual detection of maize chlorotic mottle virus using. Dissecting the mode of maize chlorotic mottle virus. Pdf characterization of maize chlorotic mottle virus associated. Coat protein gene maize chlorotic mottle virus pcr max ltd tm qpcr test 150 tests for general laboratory and research use only quantification of maize chlorotic.
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